Fig. 5

NGF attenuated TLR3-induced ROS overproduction in HCECs. a Increased generation of ROS in response to various concentrations of poly (I:C), detected by H2DCFDA probe. The fluorescence intensity was quantitatively measured as mean ± SD from three independent experiments in each condition. b-c ROS generation in cells treated with/without NGF (50 ng/ml) in response to poly (I:C) (25 μg/ml) stimulation for 10 h. C represented quantified data depicted in B. DCF fluorescence intensity was shown as fold change comparing to that of untreated cells. (d-e) NGF (50 ng/ml) treatment decreased the number of 8-OHdG positive cells in response to poly (I:C) (25 μg/ml) stimulation, detected by immunofluorescent staining . Images were representative for each of three experiments, taken at 200 × magnification