Fig. 1

Effect of Gm14461 expression on MWT and expression of Gm14461, CGRP, P2X3 receptor, P2X7 receptor, TNF-α, IL-1β, and IL-6 in TN mice. C57BL/6 J mice were randomly divided into six groups (n = 8/group): Sham, TN, TN + Scramble siRNA, TN + si-Gm14461, TN + Vector, and TN + Gm14461 group. a Gm14461 expression in mouse TGs was detected by qRT-PCR. b The MWT was measured to assess the nociception of mice at different times after CCI-ION surgery (0, 1, 3, 5, 7, 9, 11, 13, 15 d). ^*P < 0.05: Sham vs. TN; ^#P < 0.05 Scramble siRNA vs. si-Gm14461; ^$P < 0.05 Vector vs. Gm14461. c The protein levels of CGRP, P2X3 receptor, and P2X7 receptor were examined using western blot. d-f The mRNA levels of TNF-α (d), IL-1β (e), and IL-6 (f) in mouse TGs were detected by qRT-PCR. Data are presented as mean ± SD. ^*P < 0.05 vs. Sham; ^#P < 0.05 vs. Scramble siRNA; ^$P < 0.05 vs. Vector