Fig. 2
From: Mesangial cells are key contributors to the fibrotic damage seen in the lupus nephritis glomerulus
Cytokine/chemokine expression by conditionally immortalised mesangial cells following stimulation with lupus nephritis patient sera. Conditionally immortalised MCs were treated with 10% sera from patients with active (rBILAG A/B) and inactive (rBILAG D/E) LN and with age-and sex-matched HCs for 24 h. ELISA was used to assess protein levels of IL-6 in conditioned media (a) IL-8 in conditioned media (b), IL-10 in conditioned media (c), and M-CSF in conditioned media (d). IL-10 levels in sera (black bar) and conditioned media (grey bar) (e), and M-CSF levels in sera (black bar) and conditioned media (grey bar) (f). N = 5–6 per group, data are analysed using Kruskal-Wallis test with Dunn’s post hoc test, *P < 0.05 and ***P < 0.001 vs untreated MCs