Fig. 2

Effect of FGF21 deficiency on inflammatory responses in skeletal muscle of HFD-fed obese mice. (a) The levels of inflammatory cytokine (TNFα, MCP-1) mRNA in skeletal muscle from WT and FGF21-deficient mice fed an HFD. (b) NF-κB activation in skeletal muscle. NF-κB activation was determined using the p65 TransAM assay. (c) The levels of p-eIF2α/eIF2α and p-PERK/PERK protein in skeletal muscle. (d) The levels of activating transcription factor4 (ATF4) and CHOP mRNA in skeletal muscle. Results are means ± SEM (n = 4 mice per group). *P < 0.05, **P < 0.01 compared with WT mice fed an HFD