Fig. 3

Serp-2 treatment suppressed macrophage infiltration into post-ischemic infarct tissue. (a) Representative fields at 20× and 40× magnification of a sham-operated liver and from post-ischemic infarcted tissue in livers from saline or Serp-2 treated mice stained with an antibody against F4/80 and counterstained with hematoxylin. Dashed red region indicates infarcted tissue as determined by necrotic hepatocytes. Dashed yellow region indicates inflammatory cell infiltrates. Black arrows indicate F4/80-positive cells. Red arrows indicate necrotic hepatocytes. Scale bars are 100 μm (20×) and 50 μm (40×). (b) Percentages of F4/80-positive infiltrating cells per high-power field in the infarcted tissue of livers from saline or Serp-2 treated mice. Bars represent mean and standard error. Statistics calculated by Student’s T-test