Fig. 4

N44Q mutant enhanced neuronal death. N44Q mutant enhanced neuronal death: role of CD200-CD200R dysfunction. Resting or LPS stimulated BV2 cells transfected with WT or N44Q were cultured with neurons. Cortical neurons were co-cultured with BV2 cells for up to 24 h and neuronal death was estimated by LDH measurement in the media. The increased neuronal death induced by LPS stimulated BV2 cells was enhanced by the treatment with anti-CD200 antibody (^*p < 0.01, WT + LPS vs WT; ⁺p < 0.01, N44Q + LPS vs N44Q; ANOVA; n = 3). BV2 cells transfected with N44Q mutant leaded to the reinforced neuronal death (^*p < 0.01; ⁺p < 0.01; ANOVA; n = 3; ^#p < 0.05, WT + LPS + anti-CD200 vs WT + LPS; ^##p > 0.05, N44Q + LPS + anti-CD200 vs N44Q + LPS; ^&p > 0.05, WT + LPS + anti-CD200 vs N44Q + LPS). Error bars indicate ± SEM. These data suggested N44Q mutant impaired CD200-CD200R1 interaction and subsequently leaded to microglial activation and neuronal death