Fig. 2

Classical microglial activation in BV2 cells. BV2 cells were treated with LPS (100 ng/mL) or PBS for 24 h to induce M1 phenotype. BV2 cells were transiently transfected with WT or N44Q mutant of CD200R1 to illustrate the effect of mutagenesis. a, b LPS induced iNOS mRNA and CD86 mRNA and the changes between WT and N44Q mutant did not reach statistically significant difference (^*p < 0.01; ⁺p < 0.01; ^#p > 0.05, N44Q vs WT; ANOVA; n = 3). c, d IL-1β mRNA and TNF-α mRNA were upregulated when BV2 cells were treated with LPS (^*p < 0.01; ⁺p < 0.05; ^#p > 0.05, N44Q vs WT; ANOVA; n = 3). e, f Similar results were obtained by ELISA (^*p < 0.01; ⁺p < 0.05; ^#p > 0.05, N44Q vs WT; ANOVA; n = 3). Error bars indicate ± SEM