Fig. 4

Effects of TRPA1 inhibitor on mitochondrial membrane potential in IL-1β-induced rat chondrocyte. Rat chondrocytes were pre-incubated with the TRPA1 antagonist (HC-030031, 100 μM) or the vehicle for 30 min, and then stimulated with IL-1β (0, 5, 10 ng/ml) for 24 h. a Mitochondrial membrane potential fluorescence images visualized by a fluorescence microscope (200×) and the experiment was carried out in duplicate. b Mitochondrial membrane potential detected by flowcytometry and the quantitation of mitochondrial membrane potential was repeated independently three times. The results are expressed as mean ± SEM. ^*p < 0.05. TRPA1 transient receptor potential ankyrin 1, IL interleukin