Fig. 3From: Transient receptor potential ankyrin 1 (trpa1) mediates il-1β-induced apoptosis in rat chondrocytes via calcium overload and mitochondrial dysfunctionEffects of TRPA1 inhibitor on IL-1β-induced rat chondrocyte apoptosis. Rat chondrocytes were pre-incubated with the TRPA1 antagonist (HC-030031, 100 μM) or the vehicle for 30 min, and then stimulated with IL-1β (0, 5, 10 ng/ml) for 24 h. The chondrocyte apoptosis was measured by (a) Tunel staining and (b) flow cytometry. The Tunel staining was carried out in duplicate and the quantitation of apoptosis rate was repeated independently three times using flow cytometry. The results are expressed as mean ± SEM. *p < 0.05, **p < 0.01. TRPA1 transient receptor potential ankyrin 1, IL interleukinBack to article page