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Table 1 Primer sequences, product sizes and annealing temperatures used to amplify the corresponding cDNA templates. The respective longer products were used to generate standard amplification curves as described in the methods section

From: ZNF580 – a brake on Interleukin-6

Templates

Forward Primer

Reverse Primer

Product size

Annealing Temperature

ZNF580

5’ CCC TCC ACT CCT TCC T 3’

5’ ACG TGA AGG GCT TGA GGT C 3’

262 bp

60 °C

ZNF580

5’ CGC CAC CTC CTC ATC GAC GC 3’

5’ TCC GGG CAG CTG TAG CCC TT 3’

134 bp

60 °C

GAPDH

5′ TGG TGG ACC TGA CCT GCC GTC 3’

5′ AGG GGT CTA CAT GGC AAC TG 3’

415 bp

60 °C

GAPDH

5’ TCA AGA AGG TGG TGA AGC AG 3’

5’ CCC TGT TGC TGT AGC CAA AT 3’

198 bp

60 °C

IL-6

5’TGC AAT AAC CAC CCC TGA CC 3’

5′ GTG CCC ATG CTA CAT TTG CC 3’

163 bp

58 °C

IL-6

5’ TGC CAG CCT GCT GAC GAA G 3’

5’ AGC TGC GCA GAA TGA GAT GAG 3’

77 bp

58 °C