Fig. 5

Time course of ZNF580 and IL-6-production rate after LPS treatment. MonoMac6 cells were treated with 10 ng/mL LPS over 1 h to 24 h as indicated. The cells were analysed for ZNF580 mRNA by real-time PCR at seven different time points (a), (n = 3). ZNF580 protein, analysed by immunoblotting, showed an initial increase from 1 h to 8 h and a marked decrease after 16 h, representative immunoblot (b) and densitometric quantification of four independent immunoblots (c), (n = 4). The IL-6 production rate determined by ELISA was calculated as the difference of secreted IL-6 protein at two adjacent time points (d), (n = 3). Results are given as mean ± SEM, *: p ≤ 0,05