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Fig. 3 | Journal of Inflammation

Fig. 3

From: ZNF580 – a brake on Interleukin-6

Fig. 3

Increased IL-6-production after ZNF580-knockdown. Unstimulated MonoMac6 cells were transfected with scrambled siRNA or specific siRNA against ZNF580 mRNA. There was a significant knockdown of ZNF580 mRNA (a) and protein (c) at 24 h, which did not alter basal IL-6 expression (b). Transfected MonoMac6 were stimulated with 10 ng/mL LPS. The LPS-induced increase in ZNF580 was abolished when cells were transfected with siRNA against ZNF580 (d). LPS-induced IL-6 secretion was increased when the increase of ZNF580 was blocked by siRNA (e). IL-6 and ZNF580 mRNA were quantified by real-time-PCR and normalised to the corresponding GAPDH values. ZNF580 protein was analysed by immunoblotting and normalised to the corresponding β-Actin values. IL-6-protein in the supernatant was measured by ELISA. All results are shown as mean ± SEM, n = 3, *: p ≤ 0,05 (siRNA against ZNF580 versus scrambled siRNA of the respective group)

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