| | Triton X-100 | dH2O | 1 mM MSO | 10 mM MSO | 20 mM MSO | 50 mM MSO | 100 mM MSO |
|---|
OD (490 nm) | 0.800 | 0.04 | 0.04 | 0.05 | 0.03 | 0.03 | 0.03 |
% Cytotoxicity | 100 | 0 | 0 | 0 | 0 | 0 | 0 |
- Lactate dehydrogenase (LDH) activity in the medium of cells treated with 1 to 100 mM MSO. Cells were treated with concentrations of MSO ranging from 1 to 100 mM for 2 h or left untreated. Afterwards, cells were treated with Triton X-100 (positive control), or dH2O (negative control), and incubated for an additional 45 min before LDH activity in the medium was assessed. LDH activity was completely absent in the medium of cells treated with up to 100 mM MSO, indicating that MSO was not cytotoxic. 100 mM MSO had no inhibitory effect on purified, recombinant LDH (not shown)(See materials in methods for a more complete description of this assay)
