Fig. 3
From: In vitro suppression of inflammatory cytokine response by methionine sulfoximine
Effects of MSO on transcription of the cytokine genes. Mouse peritoneal macrophages were treated with MSO, then LPS as indicated for the experiments in Fig. 1 and Fig. 2. At 1, 3, or 5 h after LPS treatment, cells were, harvested, lysed, and mRNA was isolated as described in Methods. qRT-PCR was conducted using primers specific for either TNFα or IL-6. Relative mRNA levels are plotted as ΔΔCt, the log2 transformed fold increase in mRNA compared to untreated cells [13]. Data from each timepoint were obtained from at least 3 experiments, with all technical replicates shown for clarity