Fig. 2

TNFα secretion by (a) primary mouse peritoneal macrophages and (b) primary rat Kupffer cells after incubation in the presence or absence of MSO and LPS. Untreated cells, or cells treated with MSO alone (minus-LPS) did not produce detectable levels of TNFα. The level of TNFα induction by LPS stimulated controls, was normalized to 100%. A, mean TNFα protein concentration in LPS-stimulated controls was 114 pg/mL at 4 h and 183 pg/mL at 6 h. b, mean TNFα protein concentration of LPS stimulated controls was 71 pg/mL at 4 h and 117 pg/mL at 6 h. These data represent 3 peritoneal macrophage preparations and 5 Kupffer cell preparations. Each preparation contained at least 2 biological replicates for each treatment. **p < 0.01, ***p < 0.001