Fig. 7
From: MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α
Ratio of positive Th17 cells, RORγt expression, and IL-17 expression with MBD2 and HIF-1α gene silencing or overexpression a Flow cytometry used to measure the ratio of positive Th17 cells in splenic CD4+ T cells of all groups with MBD2/HIF-1α gene silencing or overexpression. b and c qRT-PCR and western blot used to measure RORγt mRNA and protein expression in all groups with MBD2 and HIF-1α gene silencing or overexpression. d Secreted IL-17 in splenic CD4+ T cells as measured using ELISA with MBD2 and HIF-1α gene silencing or overexpression. *p < 0.05 compared to control. #p < 0.05 compared with the M(+)H(+) group. $p < 0.05 compared with the M(−)H(−) group. One-way ANOVA with Bonferroni’s post hoc tests were applied to analyze the results for significant differences (*p < 0.05 or #p < 0.05 or $p < 0.05)