Fig. 6

HIF-1α expression with MBD2 gene silencing or overexpression and MBD2 expression with HIF-1α gene silencing or overexpression; HIF-1α stimulates Th17 cell differentiation and IL-17 expression a western blot to measure HIF-1α protein expression in all groups with MBD2 gene silencing (M[−]) or overexpression (M[+]). b western blot to verify HIF-1α transfection. c western blot to measure MBD2 protein expression in all groups with HIF-1α gene silencing (H[−]) or overexpression (H[+]). d Flow cytometry used to measure the ratio of positive Th17 cells in splenic CD4⁺ T cells of all groups with HIF-1α gene silencing or overexpression. e and f qRT-PCR and western blot used to measure RORγt mRNA and protein expression in all groups with HIF-1α gene silencing or overexpression. g Secreted IL-17 in splenic CD4⁺ T cells as measured using ELISA with HIF-1α gene silencing or overexpression. *p < 0.05 compared to other groups. One-way ANOVA with Bonferroni’s post hoc tests were applied to analyze the results for significant differences (*p < 0.05)