Fig. 4
From: MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α
MBD2 stimulates Th17 cell differentiation and IL-17 expression a western blot to verify MBD2 transfection. b Flow cytometry used to measure the ratio of positive Th17 cells in splenic CD4+ T cells of all groups with MBD2 gene silencing (M[−]) or overexpression (M[+]). c and d qRT-PCR and western blot used to measure RORγt mRNA and protein expression with MBD2 gene silencing or overexpression. e Secreted IL-17 in splenic CD4+ T cells measured using ELISA with MBD2 gene silencing (M[−]) or overexpression (M[+]).*p < 0.05 compared to other groups. One-way ANOVA with Bonferroni’s post hoc tests were applied to analyze the results for significant differences (*p < 0.05)