Fig. 2
From: MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α
Neutrophils-dominant asthma mediated by Th17 cells a Th17 and Th2 cells in splenocytes, stained for intracellular APC-anti-IL-17 and PE-anti-IL-4 and assessed using flow cytometry. b and c qRT-PCR and western blot used to measure RORγt and GATA3 mRNA and protein expression in all groups. d Secreted IL-17 and IL-4 in splenic CD4+ T cells using ELISA. *p < 0.05 compared to other groups. One-way ANOVA with Bonferroni’s post hoc tests were applied to analyze the results for significant differences (*p < 0.05)