Fig. 2

Effect of intratracheal LPS instillation (murine ALI model). Female C57BL/6 mice (10–12 weeks old) were challenged by intratracheal instillation of LPS (5 μg/g bodyweight), and 24 or 72 h thereafter, animals were sacrificed and, after performing bronchoalveolar lavage (BAL), the lungs were harvested. a Expression of TNF-α, MMP-9 and VEGF, quantified by RT-PCR. Relative target gene expression was normalized to 18 s RNA and is shown as fold change of expression of the PBS control (represented by the dashed line). b FACS analysis of neutrophil granulocyte count in BAL fluid (BALF; left) and in interstitial lung tissue (right). Cell numbers are expressed as percentage of all counted cells. c Albumin content was measured in BAL fluid by ELISA. ALI = Acute Lung Injury. Mean ± SD, n = 7, Mann-Whitney U test, *p < 0.05 (vs. PBS control)