Fig. 3

Hif1α was a direct target gene of miR-135a. a The predicted miR-135a binding sites on Hif1α. b and c Luciferase activity in cells cotransfected with miR-135a (or ASO-miR-135a) and luciferase reporters containing Hif1α-wild type or Hif1α-mutant type vector. The expression of Hif1α was regulated by miR-135a, according to d qRT-PCR analysis and e Western blot. miR-135a: microRNA-135a, Hif1α: Hypoxia-inducible factor 1-alpha. ns p > 0.05, ^*p < 0.05, ^**p < 0.01