Fig. 7

IL-10 ameliorated TNF-α-induced BMEC apoptosis via the STAT3 pathway. A. Assessment of BMEC apoptosis by flow cytometry (n = 3). Apoptotic signals in the Q2 and Q4 quadrants were acquired to evaluate BMEC apoptosis. B. Representative WB images of p-STAT3, STAT3, Bax and Bcl-2 protein expression in BMECs (a). WB analysis of p-STAT3/STAT3 expression (n = 3, b). WB analysis of Bax expression (n = 3, c). WB analysis of Bcl-2 expression (n = 3, d). & p < 0.001 (t-test), versus the control group, * p < 0.05 (t-test), versus the TNF-α treatment group, # p < 0.05 (t-test), versus the TNF-α + IL-10 treatment group. NS: no statistical difference