Fig. 3

IL-10 reduced BMEC apoptosis in SAP rats via the STAT3 pathway. A. TUNEL staining of BMECs in the rat brain (a); scale bar represents 200 μm. No apoptosis was observed in the control group. The SAP group exhibited numerous apoptotic BMECs and a few apoptotic glial cells. Fewer apoptotic BMECs were observed in either the IL-10 or IL-10 + S3I-201 treatment group than in the SAP group, with the IL-10 group exhibiting the fewest apoptotic BMECs. Statistical graph of BMEC apoptosis as determined by TUNEL staining (n = 8, b). B. Representative WB images of p-STAT3, STAT3, Bax and Bcl-2 protein levels in the SAP rat brain (a). WB analysis of p-STAT3/STAT3 expression (n = 8, b). WB analysis of Bax expression (n = 8, c). WB analysis of Bcl-2 expression (n = 8, d). & p < 0.001 (t-test), versus the control group, * p < 0.05 (t-test), versus the SAP group, # p < 0.05 (t-test), versus the SAP+IL-10 treatment group. NS: no statistical difference