Fig. 3

Kinetic fold changes in VNN3, ZNF746, C4BP, KNG1 and FETUB mRNA expression levels in intestinal epithelial cells after HSCT. a Kinetic fold changes in VNN3 mRNA expression in intestinal epithelial cells were consistent with protein expression in plasma. VNN3 mRNA expression in the intestinal epithelial cells in the allogeneic group peaked at the onset of GI aGVHD (day 7), and decreased during remission (day 15). Then, it increased again and peaked at day 22 when GI aGVHD relapsed. Expression at day 22 exceeded 6 times the expression at day7. Compared to the allogeneic group, VNN3 mRNA expression in the syngeneic group didn’t change significantly (P > 0.05). (a-e) VNN3, ZNF746, C4BP, KNG1 and FETUB mRNA expression peaked at day 22 when GI aGVHD recurred. Expression at day 22 was three times the expression at day 7.; ***P < 0.001. mRNA expression fold change at different time points after transplantation are normalized with those at day 7 in the allogeneic group. n = 5 mice per time point per group, two sites of intestine in each mouse was tested