Table 2 Primer sequences for real-time PCR
Name | Primer | Sequence |
|---|---|---|
β- actin | Forward | 5’- CACGATGGAGGGGCCGGACTCATC-3’ |
Reverse | 5’- TAAAGACCTCTATGCCAACACAGT-3’ | |
Nrf2 | Forward | 5’- GAGCTAGATAGTGCCCCTGG -3’ |
Reverse | 5’- CAGGACTCACGGGAACTTCT -3’ | |
SOD | Forward | 5’- AACCATCCACTTCGAGCAGA -3’ |
Reverse | 5’- GGTCTCCAACATGCCTCTCT -3’ | |
HO-1 | Reverse | 5’- CCCTAATGAGCTGCCCTACA -3’ |
Forward | 5’- TTCATCCCCACTCTCTTCGG -3’ | |
TNF-a | Forward | 5’- ACCCTCACACTCACAAACCA -3’ |
Reverse | 5’- GGCAGAGAGGAGGTTGACTT -3’ | |
IL-4 | Forward | 5’- TCTCGAATGTACCAGGAGCC-3’ |
Reverse | 5’- ACCTTGGAAGCCCTACAGAC-3’ | |
IL-6 | Forward | 5’-GTTGCCTTCTTGGGACTGAT-3’ |
Reverse | 5’-ATTAAGCCTCCGACTTGTGA-3’ | |
IL-10 | Forward | 5’- ATAACTGCACCCACTTCCCA-3’ |
Reverse | 5’- GGGCATCACTTCTACCAGGT-3’ | |
Bcl-2 | Forward | 5’- TCGCAGAGATGTCCAGTCAG-3’ |
Reverse | 5’- ATCTCCCTGTTGACGCTCTC-3’ | |
Bax | Forward | 5’-TCATGAAGACAGGGGCCTTT -3’ |
Reverse | 5’-GTCCACGTCAGCAATCATCC -3’ | |
Caspase-3 | Forward | 5’- CTACAGCACCTGGTTACTATTC-3’ |
Reverse | 5’- TACAGTTCTTTCGTGAGCAT-3’ |