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Fig. 2 | Journal of Inflammation

Fig. 2

From: Knockdown of miR-155 protects microglia against LPS-induced inflammatory injury via targeting RACK1: a novel research for intracranial infection

Fig. 2

LPS damaged mouse microglia BV2 cells in a dose-dependent manner. BV2 cells were pre-treated with different concentrations of LPS (0, 1, 5, 10, and 20 μg/ml) for 5 h. Cells without LPS treatment were used as control. a Cell viability was measured by CCK-8 assay (n = 3). b Cell apoptosis was measured by flow cytometry (n = 3). c Expression of anti-apoptotic (Bcl-2) and pro-apoptotic (Bax, caspase-3, and caspase-9) proteins were measured by western blot analysis. d Relative mRNA expressions of IL-1β, IL-6, IL-8, and TNF-α in LPS-treated cells and control cells were measured by quantitative RT-PCR (n = 3). (e-h) Concentrations of IL-1β, IL-6, IL-8, and TNF-α in LPS-treated cells and control cells were measured by ELISA (n = 3). CCK-8: Cell Counting Kit-8; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; ELISA: enzyme-linked immunosorbent assay; IL: interleukin; LPS: lipopolysaccharide; RT-PCR: reverse transcription polymerase chain reaction; TNF-α: tumor necrosis factor alpha. *P < 0.05, **P < 0.01, *** P < 0.001

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