Fig. 1

LPS damaged mouse microglia BV2 cells in a time-dependent manner. BV2 cells were pre-treated with 10 μg/ml LPS for 0, 2, 4 and 5 h. Cells without LPS treatment were used as control. a Cell viability was measured by CCK-8 assay. b Cell apoptosis was measured by flow cytometry. c-f Relative mRNA expressions of IL-1β, IL-6, IL-8, and TNF-α in LPS-treated cells and control cells were measured by quantitative RT-PCR. n = 3. CCK-8: Cell Counting Kit-8; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IL: interleukin; LPS: lipopolysaccharide; RT-PCR: reverse transcription polymerase chain reaction; TNF-α: tumor necrosis factor alpha. *P < 0.05, **P < 0.01, *** P < 0.001