Fig. 5
TMP attenuated the activation of NF-κB pathway by lipopolysaccharide exposure in microglia. (a) Cell viability was evaluated by the MTT assay. The effects of concentrations of TMP on cell viability were examined 24 h after plating of microglia. The data were collected from 6 different preparations. * P < 0.05, ** P < 0.001 vs. control. (b) Immunoblot analyses of phosphorylated (P-) IKKα/β and IKBα in cytoplasmic (Cyto) contents and p65 in nucleus (Nuc) extracts from microglia, stimulated with LPS for the indicated time periods with or without 5 μM TMP; (c) HEK293 cells (2 × 105) were transfected with the IL-6 promoter luciferase plasmid and cotransfected with expression plasmid as indicated. Cells were incubated with 5 μM TMP for 2 h before harvest. Luciferase assays were performed 36 h after transfection. Data are representative of three independent experiments. *P < 0.05