Fig. 1

Effects of the Wnt antagonists WIF1 and sFRP1 on Wnt5A-mediated phosphorylation of LIMK and CFL in VEC. Immunoblot of (a) pLIMK, LIMK and (b) pCFL and CFL in HCAEC treated with Wnt5A alone and in combination with either WIF1 or sFRP1 (sFRP) for 1 and 4 h, respectively. Bands from left to right, non-treated cells; Wnt5A-treated cells; Wnt5A + sFRP1 treated cells; Wnt5A + WIF1 treated cells. The ratios of (a) pLIMK2/LIMK2, and (b) pCFL/CFL were calculated from densitometry analyses. Data are mean ± SEM from three independent experiments. *P < 0.05 vs non-treated, **P < 0.05 vs Wnt5A