Table 1 Mouse primers used for qRT-PCR
From: Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle
Gene | Forward primer (5` → 3`) | Reverse primer (5` → 3`) |
|---|---|---|
MuRF1 | TGTCTCACGTGTGAGGTGCCTA | CACCAGCATGGAGATGCAGTTAC |
Atrogin-1 | ACATTCTGCCAGCTGCTGTTTC | TGAGTTGGATGCTGGGCCTAC |
PGC-1α | CCGTAAATCTGCGGGATGATG | CAGTTTCGTTCGACCTGCGTAA |
COX8B | AAAGCCCATGTCTCTGCCAAG | TGGAACCATGAAGCCAACGA |
NDUFA2 | ATCGTGCAACGGTACGTGGA | CCTTCAGGCTTTGCCGCTTA |
PPARα | ACGCTCCCGACCCATCTTTAG | TCCATAAATCGGCACCAGGAA |
CPT1β | GAGACAGGACACTGTGTGGGTGA | AGTGCCTTGGCTACTTGGTACGAG |
ACADM | TGATGTGGCGGCCATTAAGA | GGGTTAGAACGTGCCAACAAGAA |
ACADL | CATCCTCATGCAAGAGCTTCCACA | CTTCAAACATGAACTCACAGGCAGA |
MYL2 | AACAGAGACGGCTTCATCGACA | CGGTGAAGTTAATTGGACCTGGA |
MYH7 | GCGCAATGCAGAGTCAGTGAA | TGCAGCTTGTCCACCAGGTC |
myoglobin | TGGCAGCTGGTGCTGAATG | CCAGGGTCTCAGGGTGAGTCTTA |
TNNI1 | TCACCTGCACAGGACACGAAC | CCTTGGCCTTGGCTAGCATC |
TNNT1 | TCAATGTGCTCTACAACCGCATC | GTCATGTCCTGGCAGTCTCACTTC |
MYHC2 | TTCTCAGGCTTCAGGATTTGGTG | CTTGCGGAACTTGGATAGATTTGTG |
TNNI2 | CGAAGATCGACGTGGCTGAA | CATGGCGTCGGCAGACATAC |
TNNT3 | CATGGGTGCCAACTACAGCAG | CAGCTTGTCATCGCTAAGATGGTC |
TNNC2 | ACGGCCGCATTGACTTTGA | CTGCAGTCTGGATGGACACGA |
MYHC4 | GCTGCAGGACTTGGTGGACA | GGCCAGGTTGACATTGGATTG |
β-actin | CATCCGTAAAGACCTCTATGCCAAC | ATGGAGCCACCGATCCACA |