Fig. 3
From: Absence of 4-1BB reduces obesity-induced atrophic response in skeletal muscle
Effect of 4-1BB deficiency on expression of fiber type-related markers in the skeletal muscle of HFD-fed obese mice a qRT-PCR analysis for markers specific to slow-oxidative fibers (MYL2, MYH7, myoglobin, TNNI1, TNNT1, and MYHC2) and fast-glycolytic fibers (TNNI2, TNNT3, TNNC2, and MYHC4) in quadriceps muscles. b Immunohistochemical staining for troponin I (slow) proteins in quadriceps muscle. Arrows indicate stained cells. Magnification × 200; Scale bars, 50 μm. c troponin I (slow) and α-tubulin protein were measured by Western blotting. The relative intensities of the bands of troponin I (slow)/α-tubulin were measured using image J. Data are the means ± SEM; n = 6 in each group. **P < 0.01 when comparing the WT/HFD and KO/HFD groups