Skip to main content
Fig. 4 | Journal of Inflammation

Fig. 4

From: Successful reduction of inflammatory responses and arachidonic acid–cyclooxygenase 2 pathway in human pulmonary artery endothelial cells by silencing adipocyte fatty acid-binding protein

Fig. 4

The expression of molecules related to AA signaling cascade were down-regulated in shFABP4 HPAECs. a The protein and mRNA level of COX2 were detected by western bolt and qRT-PCR. n = 3, ***P < 0.001. b ELISA was used to examine the expression levels of PGE2. Data are presented as means ± SEM, n = 3, ***P < 0.001. c Relative intensity of AA by means of LC and MS/MS. d Standard curve line of analytical standards of AA. e The levels of AA were detected in silencing FABP4 HPAECs and normal cells stimulating by LPS. Data are presented as means ± SD, n = 3, ***P < 0.001

Back to article page