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Fig. 2 | Journal of Inflammation

Fig. 2

From: Successful reduction of inflammatory responses and arachidonic acid–cyclooxygenase 2 pathway in human pulmonary artery endothelial cells by silencing adipocyte fatty acid-binding protein

Fig. 2

Three different sequences of shRNA for silencing FABP4 transfect into HPAECs. a Western blot was used to detect the protein level of FABP4 in normal cells and cells transfected by three different sequences of shRNA and negative control duplex(named NC). β-actin was used as loading control to quantitative analysis of the relative density. Data are presented as means ± SD, n = 3, ***P < 0.001. b qRT-PCR was used to detect the most effective sequence. Data are presented as means ± SEM, n = 3, ***P < 0.001. c CCK8 was used to detect the cell viability. Data are presented as means ± SEM, n = 3

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Journal of Inflammation

ISSN: 1476-9255

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