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Fig. 1 | Journal of Inflammation

Fig. 1

From: Successful reduction of inflammatory responses and arachidonic acid–cyclooxygenase 2 pathway in human pulmonary artery endothelial cells by silencing adipocyte fatty acid-binding protein

Fig. 1

FABP4 expression is associated with the inflammation and is increased in HPAECs by LPS treatment. a The expression of TNF-α, IL-1β, IL-6 in the patients with pulmonary embolism and normal people were analyzed by ELISA. Results are shown as mean ± SD, n = 20, ***P < 0.001 compared to normal people. Western bolt was used to detect the protein level of FABP4 in cells stimulated with b) 0 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml of LPS and c) 1 μg/ml of LPS for 0 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h. β-actin was used as loading control to quantitative analysis of the relative density. Data are presented as mean ± SD, n = 3, ***P < 0.001. d qRT-PCR was used to detect FABP4 mRNA expression in cells stimulated with four different concentrations of LPS normalized to GAPDH. Data are presented as means ± SEM, n = 3, ***P < 0.001

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Journal of Inflammation

ISSN: 1476-9255

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