Fig. 1

The phenotype and phagocytic function of resident and elicited peritoneal macrophages. Fresh peritoneal macrophages were processed for phagocytosis (a-c) or cell surface markers analysis (d-h). a Phagocytosis measured by the pHrodo S.aureus bioparticles assay. b, c Phagocytosis using the Dextran-FITC and measured by flow cytometry. c Representative histogram of Flow cytometry data showing Dextran-FITC uptake by macrophages. d Mean fluorescence intensity (MFI) of F4/80. e The percentage of CD16/CD32⁺ and CD64⁺ cells in resident and elicited peritoneal macrophages. f Representative dot-plot FACS images showing CD16/CD32 and CD64 expression in resident and elicited macrophages. g & h MFI of cell surface molecule LFA-1 (g) and MHC-II (h). Mean ± SEM, N = 4 mice. *p < 0.05; **p < 0.01; ***p < 0.001. Student’s t test