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Fig. 3 | Journal of Inflammation

Fig. 3

From: Effects of corticosteroid plus long-acting beta2-agonist on the expression of PD-L1 in double-stranded RNA-induced lung inflammation in mice

Fig. 3

Poly I:C-induced upregulations of PD-L1 on lung cells were suppressed by CIC plus IND, but not by either when administered independently. Mice were anesthetized and received intratracheal instillation (i.t.) of poly I:C, 3 μg (a) or 30 μg (b). Mice were sacrificed and the lungs were collected. The lung cell suspensions were stained with FITC-conjugated anti-keratin 5/8 mAb and biotinylated anti-mouse PD-L1 mAb, followed by SAv-PE conjugate, and then processed for flow cytometry. CIC (3 μg) and/or IND (3 μg) i.t. were administered 4 h before poly I:C stimulation. The drug effects were evaluated 24 h after poly I:C stimulation, *p < 0.05 compared with stimulated control. Data is expressed as the means ± SEM of 13–24 mice

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