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Table 3 Glutathione reductase enzyme activity in the cell lysate

From: Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation

Groups

Glutathione reductase enzyme activity (nmol NADPH/min/mg of protein)

30 min

60 min

90 min

CM

0.066 ± 0.001

0.009 ± 0.0001

0.034 ± 0.01

SAM

0.113 ± 0.04a

0.263 ± 0.029a

0.355 ± 0.05a

RIBO + SAM

0.432 ± 0.05b

0.435 ± 0.04b

0.749 ± 0.090b

AZM + SAM

0.058 ± 0.005

0.066 ± 0.001b

0.118 ± 0.021b

CIP + SAM

0.035 ± 0.001b

0.423 ± 0.007b

0.073 ± 0.008b

RIBO+ AZM + SAM

0.457 ± 0.09b, c

0.491 ± 0.034b, c

1.023 ± 0.022b, d, c

RIBO+ CIP + SAM

0.265 ± 0.04b, e

0.529 ± 0.021b, d, e

1.561 ± .127b, d, e

  1. The activity of Glutathione reductase enzyme was determined following the oxidation of NADPH to NADP+ during the reduction of oxidized Glutathione and the results were expressed as nmole NADPH/min/mg of protein. Results were shown as mean ± SD of three independent experiments
  2. CM, Control macrophage, SAM S. aureus infected macrophages, RIBO + SAM Riboflavin pretreated + S. aureus infected macrophages, AZM + SAM Azithromycin treated S.aureus infected macrophage, CIP + SAM Ciprofloxacin treated S.aureus infected macrophage, RIBO + AZM + SAM Riboflavin and Azithromycin pretreated macrophages infected with S. aureus, RIBO + CIP + SAM Riboflavin and Ciprofloxacin pretreated macrophages infected with S. aureus
  3. asignificant difference with respect to CM
  4. bsignificant difference with respect to SAM
  5. csignificant difference with respect to ciprofloxacin (CIP)
  6. dsignificant difference with respect to Riboflavin(RIBO) plus S. aureus infected macrophages at P < 0.05 level of significance
  7. eSignificant difference with respect to Azithromycin (AZM) plus S. aureusinfected macrophages