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Table 2 Determination of H2O2, Superoxide anion and nitric oxide (NO) released from different control sets

From: Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation

Groups

H2O2 (μM/106cell)

Superoxide anion (nM/106cells)

Nitric oxide (mM/106cells)

30MINS

60MINS

90MINS

30MINS

60MINS

90MINS

30MINS

60MINS

90MINS

Control Mφ

0.85 ± 0.05

0.17 ± 0.03

0.24 ± 0.02

1.25 ± 0.004

0.90 ± 0.002

0.91 ± 0.002

0.69 ± 0.002

0.71 ± 0.001

0.53 ± 0.018

Control Mφ + RIBO

1.16 ± 0.1a

2.41 ± 0.05a

2.51 ± 0.08

1.7 ± 0.06a

1.96 ± 0.01a

2.31 ± 0.0a

0.530 ± 0.018

0.45 ± 0.00a

0.40 ± 0.001

Control Mφ + AZM

0.77 ± 0.01

0.53 ± 0.01a

0.17 ± 0.03

1.16 ± 0.12

1.58 ± 0.02a

2.07 ± 0.002a

0.32 ± 0.017a

0.53 ± 0.01

0.77 ± 0.01a

Control Mφ + CIP

1.16 ± 0.1a

1.54 ± 0.02a

1.90 ± 0.08a

1.25 ± 0.004

0.96 ± 0.01

2.15 ± 0.02a

0.72 ± 0.002a

0.85 ± .0.05

0.913 ± 0.01a

  1. H2O2, superoxide anion and NO were determined from the control macrophages either alone or in combination with RIBO, AZM or CIP. Results were shown as mean ± SD of three independent experiments
  2. CM Control macrophage, CM + RIBO Riboflavin pre-treated Control macrophage, AZM + CM Azithromycin pretreated control macrophage, CIP + CM Ciprofloxacin pretreated control macrophage
  3. asignificant difference with respect to CM