Fig. 6
Effect of Riboflavin and antibiotic treatment (AZM and CIP) on S. aureus infection induced alteration on the activity of catalase, SOD enzyme and GSH, LPO level in the lysate of murine peritoneal macrophages. The lysate was recovered after time-dependent phagocytosis in the presence or absence of RIBO or antibiotic treatment, as described in the “Methods and materials” section, and were used to determine the catalase (a), SOD enzyme activity (b), GSH level (c), and LPO content (d) in the supernatant. Catalase enzyme activity was measured in the presence of 15 μmoles of H2O2/mLof phosphate buffer and is expressed in terms of mmol/min mg protein (a); SOD enzyme activity was expressed in terms of SOD units/mg protein (b); GSH activity was expressed in terms of μM/mg protein (c); and LPO content was expressed in terms of nmol of thiobarbituric acid-reactive substances (TBARS) (d) Results were shown as mean ± SD of three independent experiments. CM, Control macrophage; SAM, S. aureus infected macrophages; RIBO + SAM, Riboflavin pretreated + S. aureus infected macrophages; AZM + SAM, Azithromycin treated S.aureus infected macrophage; CIP + SAM, Ciprofloxacin treated S.aureus infected macrophage; RIBO + AZM + SAM, Riboflavin and Azithromycin pretreated macrophages exposed to S. aureus. RIBO + CIP + SAM, Riboflavin and Ciprofloxacin pretreated macrophages exposed to S. aureus; &, significant difference with respect to CM *, significant difference with respect to SAM; #, significant difference with respect to Riboflavin (RIBO) plus S. aureus infected macrophages at P < 0.05 level of significance. ^, significant difference with respect tociprofloxacin (CIP) or % Significant difference with respect to Azithromycin (AZM) plus S. aureus infected macrophages