Fig. 3
Effect of Riboflavin and antibiotic treatment on S. aureus infection induced alteration in the H2O2 release and superoxide anion release in the lysate of murine peritoneal macrophages. The lysate recovered after time dependent phagocytosis in presence or absence of Riboflavin or antibiotics were used to determine the H2O2 (a) and superoxide anion release (b) and was expressed in terms of μM/106cell and nM/106cells. Results were presented as mean ± SD of three independent experiments. CM, Control macrophage; SAM, S. aureus infected macrophages; RIBO + SAM, Riboflavin pretreated + S. aureus infected macrophages; AZM + SAM, Azithromycin treated S.aureus infected macrophage; CIP + SAM, Ciprofloxacin treated S.aureus infected macrophage; RIBO + AZM + SAM, Riboflavin and Azithromycin pretreated macrophages exposed to S. aureus. RIBO + CIP + SAM, Riboflavin and Ciprofloxacin pretreated macrophages exposed to S. aureus; &, significant difference with respect to CM *, significant difference with respect to SAM; #, significant difference with respect to Riboflavin(R) plus S. aureus infected macrophages at P < 0.05 level of significance.^, significant difference with respect to ciprofloxacin (CIP) or % Significant difference with respect to Azithromycin (AZM) plus S. aureus infected macrophages