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Fig. 1 | Journal of Inflammation

Fig. 1

From: Chondroprotective effect of high-molecular-weight hyaluronic acid on osteoarthritic chondrocytes in a co-cultivation inflammation model with M1 macrophages

Fig. 1

Establishment and verification of a functional in vitro macrophage inflammatory model. a Verification of the differentiation from THP-1 cells to resting M0 macrophages (rM0) and proinflammatory M1 macrophages via flow cytometry analysis of CD14 cell surface expression. b Representative RT-qPCR gene expression of M1-specific macrophage genes in rM0 and M1 macrophages (with or without the addition of the anti-inflammatory glucocorticoid dexamethasone (Dex)). c Analysis of secreted pro-inflammatory cytokines into the supernatant after rM0 and M1 culture (with or without the addition of the anti-inflammatory glucocorticoid dexamethasone (Dex))

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