Fig. 6

Fluorescence micrographs of GPR18 immunoreactivity. Neutrophils on glass coverslips were fixed with 2 % paraformaldehyde in HBSS and stained with anti-GPR18 antibody (right panel) or isotype control (left panel), then washed and incubated with the secondary FITC-conjugated detection antibody (green). Cells were mounted with Hoechst 33258 (Sigma-Aldrich, Oakville, Ontario, Canada) (blue). No immunoreactivity was detected in the control cells, but most neutrophils expressed GPR18. Scale bar = 100 μm