Fig. 5

The effects of Abn-CBD on neutrophil recruitment assessed using a parallel plate flow chamber assay. HUVEC monolayers were activated with TNF-α (10 nM) 4 h prior to the experiment. a Abn-CBD was added to the HUVEC for 1 h and freshly isolated neutrophils were perfused over the monolayer for 4 min. b Using a different approach neutrophils were treated with Abn-CBD and O-1918 for 15 min and then perfused over the HUVEC monolayer, neutrophil accumulation was then assessed after 4 min of perfusion, and c transmigration of neutrophils through the monolayer was determined after 6 min. Neutrophil accumulation (after 4 min) and transmigration (after 6 min) was significantly decreased, when neutrophils were incubated with Abn-CBD. *, p < 0.05 and **, p < 0.01 when compared to control, Abn-CBD + O-1918 and O-1918; n = 5/group