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Fig. 4 | Journal of Inflammation

Fig. 4

From: Abnormal cannabidiol attenuates experimental colitis in mice, promotes wound healing and inhibits neutrophil recruitment

Fig. 4

The effects of Abn-CBD on neutrophil chemotaxis. Neutrophils were incubated with Abn-CBD and/or O-1918 and pipetted into the top chamber of 5 μm Transwells (2 × 105/well). Migration towards IL-8 (10 nM) was assessed after 3 h by counting adherent neutrophils at the bottom of the chamber (3 non-overlapping fields/well). The open bar depicts random migration without an IL-8 stimulus. Abn-CBD significantly reduced neutrophil chemotaxis, an effect that was blocked by O-1918. Data were analyzed with one-way ANOVA with Tukey’s post-hoc test. *, p < 0.05 when compared to control; n = 3/group

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