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Fig. 6 | Journal of Inflammation

Fig. 6

From: Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells

Fig. 6

Effect of S2505 on the expression of PPAR-γ and ET-1 mRNA. A549 cells were incubated with 10 ng/mL TGF-β1 or 10 μM S2505 respectively while cells were pre-treated with 10 μM S2505 for 60 min before 10 ng/mL TGF-β1 stimulation for the S2505 + TGF-β1 group. Then the samples were collected and analyzed by real-time qPCR, ELISA and western blotting. The relative PPAR-γ mRNA level (normalized to GAPDH mRNA) in S2505 group was significantly increased (a) while compared to control group and the ET-1 level was significantly decreased (b) (*p < 0.05). The increased expression of ET-1 mRNA (c) and protein (d) mediated by TGF-β1was suppressed by S2505 (#p < 0.05). Western blotting showed that S2505 decreased the expression of phospho-P38 and phospho-Smad2 (e). The results are presented as means ± SD of 3 independent experiments performed in triplicate. (C, control group; S2505, S2505 group; TGF, TGF-β1 group; S2505 + TGF, S2505 + TGF-β1 group)

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