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Fig. 3 | Journal of Inflammation

Fig. 3

From: Transforming growth factor beta 1 induced endothelin-1 release is peroxisome proliferator-activated receptor gamma dependent in A549 cells

Fig. 3

TGF-β1 mediated Smad2 phosphorylation and nuclear transfer. Confluent A549 cells were treated with 10 ng/mL TGF-β1 and cell lysates were subjected to western blotting analysis. For immunofluorescence analysis by CLSM, the cells were pre-treated without or with 10 μM SB203580 and then incubated with 10 ng/mL TGF-β1 and fixed in 4 % paraformaldehyde, treated as described in the Materials and Methods and the cells were observed and photographed by a confocal laser scanning microscope. The red fluorescence stained for cell nucleus and green fluorescence stained for phosphor-Smad2. Western blotting showed that TGF-β1 increased the phosphorylation expression of Smad2 (a). The pictures of CLSM demonstrated that TGF-β1 mediated Smad2 nuclear transfer (b), this process was suppressed by SB203580 (c) (original magnification × 400)

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