Table 1 Real-time quantitative PCR: Cell types, primers and results

Human PBMCs

Taqman primer Hs00168362_m1

GAPDH: Taqman primer Hs02758991_g1

Very low or absent

(see Methods)

Hprt1: Taqman primer Hs02800695_m1

ActB: Taqman primer Hs1060665_g1

RPLP0: Taqman primer Hs004189669_g1

Human T cell populations:

Taqman primer Hs00168362_m1

GAPDH: Taqman primer Hs02758991_g1

Very low or absent in all T cell subpopulations

-CD3+

-CD4+

-CD8+

-CD4+ CD25+

(total RNA obtained from Miltenyi Biotec; Cat. nos: 130-093-165, 164, 163 and 168)

Human primary monocytes (see Methods)

Taqman primer Hs00168362_m1

GAPDH: Taqman primer Hs02758991_g1

Expressed

Hprt1: Taqman primer Hs02800695_m1

Human immature dendritic cells (total RNA obtained from 3H Biomedical; Cat. no: 3H100-70-5)

Taqman primer Hs00168362_m1

GAPDH: Taqman primer Hs02758991_g1

Expressed

Hprt1: Taqman primer Hs02800695_m1

ActB: Taqman primer Hs1060665_g1

RPLP0: Taqman primer Hs00420895_gH

Human THP-1 monocytes and macrophages (phorbol 12-myristate 13-acetate-differentiated)

Taqman primer Hs00168362_m1

GAPDH: Taqman primer Hs02758991_g1

Expressed under inflammatory conditions

Hprt1: Taqman primer Hs02800695_m1

ActB: Taqman primer Hs1060665_g1

RPLP0: Taqman primer Hs00420895_gH

Mouse peritoneal macrophages (thioglycolate-elicited, see Methods)

Taqman primer Mm00434123_m1

GAPDH: Taqman primer Mm99999915_g1

Expressed

Hprt1: Taqman primer Mm00446968_m1