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Table 1 Real-time quantitative PCR: Cell types, primers and results

From: A human and animal model-based approach to investigating the anti-inflammatory profile and potential of the 5-HT2B receptor antagonist AM1030

Cell type HTR2B primers Reference gene primer HTR2B expression
Human PBMCs Taqman primer Hs00168362_m1 GAPDH: Taqman primer Hs02758991_g1 Very low or absent
(see Methods)
Hprt1: Taqman primer Hs02800695_m1
ActB: Taqman primer Hs1060665_g1
RPLP0: Taqman primer Hs004189669_g1
Human T cell populations: Taqman primer Hs00168362_m1 GAPDH: Taqman primer Hs02758991_g1 Very low or absent in all T cell subpopulations
-CD3+
-CD4+
-CD8+
-CD4+ CD25+
(total RNA obtained from Miltenyi Biotec; Cat. nos: 130-093-165, 164, 163 and 168)
Human primary monocytes (see Methods) Taqman primer Hs00168362_m1 GAPDH: Taqman primer Hs02758991_g1 Expressed
Hprt1: Taqman primer Hs02800695_m1
Human immature dendritic cells (total RNA obtained from 3H Biomedical; Cat. no: 3H100-70-5) Taqman primer Hs00168362_m1 GAPDH: Taqman primer Hs02758991_g1 Expressed
Hprt1: Taqman primer Hs02800695_m1
ActB: Taqman primer Hs1060665_g1
RPLP0: Taqman primer Hs00420895_gH
Human THP-1 monocytes and macrophages (phorbol 12-myristate 13-acetate-differentiated) Taqman primer Hs00168362_m1 GAPDH: Taqman primer Hs02758991_g1 Expressed under inflammatory conditions
Hprt1: Taqman primer Hs02800695_m1
ActB: Taqman primer Hs1060665_g1
RPLP0: Taqman primer Hs00420895_gH
Mouse peritoneal macrophages (thioglycolate-elicited, see Methods) Taqman primer Mm00434123_m1 GAPDH: Taqman primer Mm99999915_g1 Expressed
Hprt1: Taqman primer Mm00446968_m1