Fig. 2
Influence of the 5-HT2B antagonist AM1030 on LPS-induced cytokines in human monocytes and mouse macrophages. In (a) and (b), the effect of AM1030 and the reference 5-HT2B receptor antagonist RS127445 on the production of TNF (a) and IL-6 (b) by LPS-stimulated human primary monocytes. Data resulting from three healthy blood donors were pooled by normalizing sample cytokine levels to untreated control and are presented as percent of LPS control. The absolute levels (mean ± SD) of TNF and IL-6 were 483 ± 13 pg/ml and 230 ± 84 pg/ml, respectively. In (c) and (d), the influence of AM1030 on the production of IL-6 by human THP-1 monocytes (c) and mouse peritoneal macrophages (d) is shown. One sample t-test (a and b) and one-way ANOVA with Dunnett’s post test (c and d) were used for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001)