Fig. 6

EPO administration increases the expression levels of EPO receptors in ischemic limbs. Twenty-four hours after CLI, quadriceps from ischemic limbs were isolated for cryosections and used for immunohistochemical staining to detect the expression and distribution of EPO receptor (EPOR). a-e The endothelial cells were recognized with antibodies against CD31 and shown in green color. The expression of EPOR was recognized with antibodies against EPOR and shown in red color. Arrows indicated the localization of EPOR positively stained (EPOR+) cells in quadriceps. f The quantitation of the number of EPOR+ cells. Statistical analysis used one-way ANOVA followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡, §) indicate significance at p value less than .05