Fig. 5

Expression and phosphorylation levels of Akt, JNK, and ERK1/2 in ischemic quadriceps. a Total and phosphorylated protein expression levels of Akt, JNK, and ERK1/2 were examined through Western blots with specific antibodies. b Expression levels of total Akt. c Expression levels of phosphorylated Akt. d Expression levels of total JNK. e Expression levels of phosphorylated JNK. f Expression levels of total ERK1/2. g Expression levels of phosphorylated ERK1/2. The protein expression levels are quantitated and normalized with the expression levels of β-tubulin. Statistical analysis used one-way ANOVA followed by Bonferroni multiple comparison post hoc test (n = 8 for each group). Symbols (*, †, ‡) indicate significance at p value less than 0.05